![]() In adulthood, rats were trained on a delayed-match-to-sample working memory task which requires responding after increasingly difficult delays. Methods: Adolescent male and female rats self-administered escalating doses of THC (3, 10, 30, and 100 µg/kg/infusion) alone or combined with CBD (10:1 ratio). We also investigated the protracted and acute effects of THC on prefrontal cortical activity during the cognitive task. We investigated the impact of CBD on intravenous THC self-administration in adolescent male and female rats and establish if it protected against diminished performance on a cognitive task. Cannabidiol (CBD) is a component of cannabis that may diminish the aversive properties of THC during self-administration or protect against cognitive impairments associated with drug. Sierra Stringfield University of Pittsburgh, Pittsburgh, Pennsylvania, United Statesīackground: Cannabis use during adolescence is associated with cognitive deficits and risk of psychiatric disorders in adulthood. Cannabinoid withdrawal was more robust in male rats in the non-contingent model, contrary to our expectations. Equivalent analysis was performed after WIN SA.Ĭonclusions: There is a robust spontaneous cannabinoid withdrawal syndrome observed following spontaneous withdrawal from experimenter-administered or self-administered SCB in male and female rats. Tukey’s multiple comparison testing indicated a significant difference between WIN treated male and female rats at 48 hours with males showing a higher GWS (p = 0.0415). There was a main effect of WIN treatment (F(1,41) = 22.15, p < 0.0001) and a Time x Sex interaction (F(5,119) = 2.502, p = 0.0342) on GWS with spontaneous withdrawal from experimenter administered WIN injections. ![]() Sidak’s multiple comparison testing revealed a significant WIN treatment effect in males only (p = 0.035). Results: There was a main effect of WIN treatment on GWS (F(1,26) = 6.611, p = 0.0162) with precipitated withdrawal from experimenter administered WIN. Somatic withdrawal signs were observed over 30 minutes to generate a global withdrawal score (GWS). Withdrawal was precipitated with SR 141716A four hours after the final SA session (n = 3-4/group), and spontaneous withdrawal was scored at 14, 24, 48, 72, and 96 hours (n = 3-6/group). In the self-administration studies (SA), rats were given access to WIN (6.25 ug/kg for females 12.5 ug/kg for males) or vehicle under standard FR1 SA parameters. Spontaneous withdrawal was observed at 6, 14, 24, 48, 72, and 96 hours (n = 7-8/group). Withdrawal was precipitated with the CB1R inverse agonist SR 141716A (10 mg/kg, ip) four hours after the final infusion (n = 8/group). ![]() In experimenter-administered drug studies, rats were given escalating doses of WIN (0.2, 0.4, 0.6, and 0.8 mg/kg) via twice daily iv infusions (vehicle injected animals as controls). Methods: Adult male and female Long Evans rats received jugular catheter implantation. This study assessed withdrawal in male and female rats following two methods of administration of the SCB WIN. SCBs produce significant adverse effects, and users of SCBs report more severe withdrawal compared to people using plant cannabis. WIN55,212-2 (WIN) is an aminoalkylindole derivative, and drugs from this class are common in SCB products. Sade Spencer The University of Minnesota, Minneapolis, Minnesota, United Statesīackground: Synthetic cannabinoids (SCBs) gained popularity as a designer drug alternative to cannabis in the 2000s.
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